Transglutaminase 2 anticorps

N° du produit ABIN3025598

Cet anticorps Souris Monoclonal détecte spécifiquement Transglutaminase 2 dans IF, IHC (p) et FACS. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Singe et Lapin.

Aperçu rapide pour Transglutaminase 2 anticorps (ABIN3025598)

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivité: Humain, Souris, Rat, Singe, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transglutaminase 2 est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: TGM2-419

Détail du produit anti-Transglutaminase 2 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant full-length human protein was used as the immunogen for the Transglutaminase 2 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the Transglutaminase 2 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Transglutaminase 2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Transglutaminase 2

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Autre désignation: Transglutaminase 2

Sujet: Recognizes a 77-85  kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling