CD44 anticorps

N° du produit ABIN3025607

Cet anticorps Souris Monoclonal détecte spécifiquement CD44 dans FACS, WB, IF, IHC (p) et Func. Il présente une réactivité avec des échantillons de Humain, Babouin et Green Monkey.

Aperçu rapide pour CD44 anticorps (ABIN3025607)

Antigène: CD44

Reactivité: Humain, Babouin, Green Monkey

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD44 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Clone: SPM544

Détail du produit anti-CD44 anticorps

Purification: Protein G affinity chromatography

Immunogène: Stimulated human leukocytes were used as the immunogen for this anti-CD44 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: The optimal dilution of the anti-CD44 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Functional Studies: Order Ab without BSA/azide,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD44

Antigène: CD44

Autre désignation: CD44

Sujet: Recognizes a cell surface glycoprotein of 80-95  kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95  kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling