PCNA anticorps

N° du produit ABIN3025663

Cet anticorps anti-PCNA Monoclonal Souris (Clone SPM350) (ABIN3025663) détecte spécifiquement PCNA dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour PCNA anticorps (ABIN3025663)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: SPM350

Détail du produit anti-PCNA anticorps

Fonction: Anti-PCNA Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogène: Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy