GRP94 anticorps

N° du produit ABIN3025969

Cet anticorps anti-GRP94 est un anticorps Monoclonal produit chez le Rat, détectant GRP94 dans WB, FACS, IF et IHC (p). Convient pour Humain, Souris, Rat, Poulet, Boeuf (Vache), Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval et Xenopus laevis.

Aperçu rapide pour GRP94 anticorps (ABIN3025969)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris, Rat, Poulet, Boeuf (Vache), Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval, Xenopus laevis

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 9G10-F8-2

Détail du produit anti-GRP94 anticorps

Purification: Protein G affinity chromatography

Immunogène: Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the GRP94 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistology (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: GRP94

Sujet: Recognizes a protein of 94  kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades