GRP94 anticorps

N° du produit ABIN3025969

Cet anticorps anti-GRP94 Monoclonal Rat (Clone 9G10-F8-2) (ABIN3025969) détecte spécifiquement GRP94 dans WB, FACS, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour GRP94 anticorps (ABIN3025969)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris, Rat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: 9G10-F8-2

Détail du produit anti-GRP94 anticorps

Fonction: GRP94 Antibody / HSP90B1 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogène: Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal dilution of the GRP94 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: GRP94

Sujet: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades