TGFA anticorps (AA 34-43)

N° du produit ABIN3026449

Cet anticorps anti-TGFA est un anticorps Souris Monoclonal détectant TGFA dans IF, FACS et IHC (p). Adapté pour Humain, Lapin et Poisson zèbre (Danio rerio).

Aperçu rapide pour TGFA anticorps (AA 34-43) (ABIN3026449)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain, Lapin, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: P-T1

Détail du produit anti-TGFA anticorps

Épitope: AA 34-43

Attributs du produit: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Purification: Protein G affinity chromatography

Immunogène: Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the TGF-alpha antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the TGF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF-alpha

Sujet: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway