CDK1 anticorps

N° du produit ABIN3026474

Cet anticorps Souris Monoclonal détecte spécifiquement CDK1 dans IF, IHC (p) et FACS. Il présente une réactivité avec des échantillons de Humain, Singe, Boeuf (Vache) et Vison.

Aperçu rapide pour CDK1 anticorps (ABIN3026474)

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivité: Humain, Singe, Boeuf (Vache), Vison

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK1 est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: POH-1

Détail du produit anti-CDK1 anticorps

Aucune reactivité croisée: Souris, Rat (Rattus), Xenopus laevis, Mouche des fruits (Drosophila melanogaster), Schizosaccharomyces pombe, Yeast (Saccharomyces cerevisiae)

Attributs du produit: This antibody recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Purification: Protein G purified antibody

Immunogène: Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this Cdk1 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdk1 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE) only: 1-2 μg/mL (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cdk1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CDK1

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: Cdk1

Sujet: This antibody recognizes a 34  kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

ID gène: 983

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA