PCNA anticorps

N° du produit ABIN3026537

Cet anticorps Souris Monoclonal détecte spécifiquement PCNA dans WB, ELISA, IF, FACS, IHC (p) et IP. Il présente une réactivité envers Humain, Souris, Rat, Poulet, Singe, Porc, Saccharomyces cerevisiae, Poisson zèbre (Danio rerio), Drosophila melanogaster et Schizosaccharomyces pombe.

Aperçu rapide pour PCNA anticorps (ABIN3026537)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Poulet, Singe, Porc, Saccharomyces cerevisiae, Poisson zèbre (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: PC10

Détail du produit anti-PCNA anticorps

Attributs du produit: This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

Purification: Protein G affinity chromatography

Immunogène: Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: This antibody recognizes a non-histone protein of 36  kDa, which is identified as proliferating cell nuclear antigen (PCNA). PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

ID gène: 5111

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy