TJP3 anticorps (C-Term)

N° du produit ABIN3029137

Cet anticorps Lapin Polyclonal détecte spécifiquement TJP3 dans WB et IHC (p). Il présente une réactivité envers Rat et Souris.

Aperçu rapide pour TJP3 anticorps (C-Term) (ABIN3029137)

Antigène: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TJP3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TJP3 anticorps

Épitope: C-Term

Purification: Antigen affinity

Immunogène: An amino acid sequence from the C-terminus of mouse Zonula occludens protein 3 (RARDVESSDDEGYDWGPATDL) was used as the immunogen for this ZO-3 antibody (100% rat homology).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the ZO-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the ZO-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur TJP3

Antigène: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Autre désignation: ZO-3

Sujet: TJP3 (Tight junction protein 3), also called ZO-3 (Zona occludens 3), is a protein that in humans is encoded by the TJP3 gene. TJP3/ZO-3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999). Haskins et al.(1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds directly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al.(2002) determined that Tjp3 interacted with PATJ(INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.

UniProt: Q9QXY1