GRP94 anticorps

N° du produit ABIN303101

L’anticorps anti-GRP94 Polyclonal Lapin est utilisé pour la détection de GRP94 dans des échantillons de Humain, Souris, Rat, Poulet, Singe et Drosophila melanogaster. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour GRP94 anticorps (ABIN303101)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris, Rat, Poulet, Singe, Drosophila melanogaster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-GRP94 anticorps

Specificité: Amino acids 289-300 of human Hsp90 protein.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide.

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Western Blot (1/500-1/2500). ELISA (1/5000-1/25000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: Endoplasmin / HSP90B1 / TRA1

Sujet: Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo, and interacts with TOM34, AHSA1, HDAC6 and SMYD3.Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1

ID gène: 7184

NCBI Accession: NP_003290

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades