MAG anticorps (AA 114-132)

N° du produit ABIN3031888

Détail du produit anti-MAG anticorps

Antigène: MAG (Myelin Associated Glycoprotein (MAG))

Épitope: AA 114-132

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogène: Amino acids 114-132 (KYYFRGDLGGYNQYTFSEH) were used as the immunogen for this MAG antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the MAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. MAG is seen in western blotting from 60~100KD depending on the level of glycosylation.\. Western blot: 0.5-1 μg/mL (1),IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the MAG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur MAG

Antigène: MAG (Myelin Associated Glycoprotein (MAG))

Autre désignation: MAG (MAG Produits)

Synonymes: anticorps GMA, anticorps S-MAG, anticorps SIGLEC-4A, anticorps SIGLEC4A, anticorps siglec-4, anticorps MAG, anticorps Gma, anticorps siglec-4a, anticorps myelin associated glycoprotein, anticorps myelin-associated glycoprotein, anticorps MAG, anticorps mag, anticorps Mag

Sujet: Myelin-associated glycoprotein, also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. Resistant embryonic neurons were rendered sensitive by expression of NgR. The glycoprotein binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with the glycoprotein prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway