NLRP3 anticorps (N-Term)

N° du produit ABIN3032021

L’anticorps Lapin Polyclonal anti-NLRP3 a été validé pour WB, IF, FACS, IHC (p) et ICC. Il convient pour détecter NLRP3 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NLRP3 anticorps (N-Term) (ABIN3032021)

Antigène: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NLRP3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-NLRP3 anticorps

Épitope: N-Term

Purification: Antigen affinity

Immunogène: An amino acid sequence from the N-terminus of human NLRP3 (RYLEDLEDVDLKKFKMHLED) was used as the immunogen for this NLRP3 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the NLRP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IF/ICC: 2-3 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the NLRP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur NLRP3

Antigène: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Autre désignation: NLRP3 (NALP3)

Sujet: NOD-like receptor family pyrin domain containing 3, also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. The gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the proteins nucleotide-binding domain reduced ATP binding, Caspase-1 activation, IL-1b production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for NLRP3 inflammasomes in antifungal immunity, Gross et al.showed that deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome