14-3-3 sigma/SFN anticorps (AA 140-156)

N° du produit ABIN3032756

L’anticorps Lapin Polyclonal anti-14-3-3 sigma/SFN a été validé pour WB, IHC (p) et ICC. Il convient pour détecter 14-3-3 sigma/SFN dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour 14-3-3 sigma/SFN anticorps (AA 140-156) (ABIN3032756)

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 sigma/SFN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-14-3-3 sigma/SFN anticorps

Épitope: AA 140-156

Purification: Antigen affinity

Immunogène: Amino acids 140-156 (KKRIIDSARSAYQEAMD) were used as the immunogen for this 14-3-3 sigma antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the 14-3-3 sigma antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the 14-3-3 sigma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur 14-3-3 sigma/SFN

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Autre désignation: 14-3-3 sigma

Sujet: Stratifin (SFN), also known as 14-3-3 sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993) obtained a peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein called stratifin was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

UniProt: P31947

Pathways: Signalisation p53, Myometrial Relaxation and Contraction