TRIM5 anticorps (N-Term)

N° du produit ABIN303309

Cet anticorps anti-TRIM5 est un anticorps Lapin Polyclonal détectant TRIM5 dans WB et IHC (p). Adapté pour Humain.

Aperçu rapide pour TRIM5 anticorps (N-Term) (ABIN303309)

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TRIM5 anticorps

Épitope: N-Term

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic Peptide corresponding to Amino Acids near the mid-region of human TRIM5a.

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunohistochemistry (2 μg/mL). Western Blot (5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate Buffered Saline PBS containing 0.02 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Détails sur TRIM5

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Autre désignation: TRIM5 / RNF88

Sujet: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the a variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5a variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5a variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5a acts as a natural barrier to cross-species retrovirus transmission. While a function has not yet been assigned to TRIM5g, it is known that expression of TRIM5a variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. Furthermore, the TRIM5d isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner. The TRIM5d isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5d also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.Synonyms: RING finger protein 88, Tripartite motif-containing protein 5

ID gène: 85363

NCBI Accession: NP_149023

UniProt: Q9C035

Pathways: Activation of Innate immune Response