Nestin anticorps (AA 1484-1500)

N° du produit ABIN303340

Cet anticorps Lapin Polyclonal détecte spécifiquement Nestin dans WB, IF, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Hamster, Lapin et Macaque.

Aperçu rapide pour Nestin anticorps (AA 1484-1500) (ABIN303340)

Antigène: Nestin (NES)

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Hamster, Lapin, Macaque

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Nestin anticorps

Épitope: AA 1484-1500

Specificité: This antibody recognizes Nestin.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Dog, Hamster, Macaque, Rabbit, RatSpecies reactivity (tested):Human, Mouse.

Purification: Immunoaffinity Chromatography

Immunogène: Recombinant protein corresponding to amino acids 1484-1500 of Human Nestin.

Information d'application

Indications d'application: ELISA (1/20000-1/80000). Immunofluorescence. Western Blot (1/500-1/3000). Immunohistochemistry on Paraffin Sections (5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin

Sujet: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.Synonyms: NES, Nbla00170

ID gène: 10763

NCBI Accession: NP_006608

UniProt: P48681