GLI3 anticorps (AA 41-57)

N° du produit ABIN303380

L’anticorps anti-GLI3 Polyclonal Lapin est utilisé pour la détection de GLI3 dans des échantillons de Humain, Rat, Poulet, Chien, Singe, Caille et Grenouille. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour GLI3 anticorps (AA 41-57) (ABIN303380)

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivité: Humain, Rat, Poulet, Chien, Singe, Caille, Grenouille

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-GLI3 anticorps

Épitope: AA 41-57

Specificité: This antibody recognises GLI-Kruppel Family Member GLI3.

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee, Chicken, Canine (Dog), Frog, Monkey, Quail and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogène: Synthetic Peptide -KLH conjugated corresponding to Amino acids 41-57 of Human Gli-3 protein.

Information d'application

Indications d'application: ELISA (1/6000-1/30000). Western Blot (1/500-1/2000). Immunohistochemistry on Paraffin Sections (1.25 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Détails sur GLI3

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: GLI3

Sujet: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.Synonyms: GLI-3, Zinc finger protein GLI3

ID gène: 2737

NCBI Accession: NP_000159

UniProt: P10071

Pathways: Signalisation Hedgehog