GLI3 anticorps (AA 41-57)
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- Antigène Voir toutes GLI3 Anticorps
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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Épitope
- AA 41-57
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Reactivité
- Humain, Rat, Chien, Poulet, Singe, Caille, Grenouille
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp GLI3 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificité
- This antibody recognises GLI-Kruppel Family Member GLI3.
- Réactivité croisée (Details)
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Species reactivity (expected):Chimpanzee, Chicken, Canine (Dog), Frog, Monkey, Quail and Rat.
Species reactivity (tested):Human. - Purification
- Immunoaffinity Chromatography
- Immunogène
- Synthetic Peptide -KLH conjugated corresponding to Amino acids 41-57 of Human Gli-3 protein.
- Top Product
- Discover our top product GLI3 Anticorps primaire
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- Indications d'application
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ELISA (1/6000-1/30000). Western Blot (1/500-1/2000). Immunohistochemistry on Paraffin Sections (1.25 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid cycles of freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.
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- Antigène
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- Autre désignation
- GLI3 (GLI3 Produits)
- Synonymes
- anticorps ACLS, anticorps GCPS, anticorps GLI3-190, anticorps GLI3FL, anticorps PAP-A, anticorps PAPA, anticorps PAPA1, anticorps PAPB, anticorps PHS, anticorps PPDIV, anticorps xgli3, anticorps AI854843, anticorps AU023367, anticorps Bph, anticorps Pdn, anticorps Xt, anticorps add, anticorps im:7142603, anticorps GLI3, anticorps GLI family zinc finger 3, anticorps GLI family zinc finger 3 L homeolog, anticorps GLI-Kruppel family member GLI3, anticorps GLI3, anticorps Gli3, anticorps gli3.L, anticorps gli3
- Sujet
- Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.Synonyms: GLI-3, Zinc finger protein GLI3
- ID gène
- 2737
- NCBI Accession
- NP_000159
- UniProt
- P10071
- Pathways
- Signalisation Hedgehog
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