KIT Ligand anticorps (N-Term)

N° du produit ABIN3042665

L’anticorps Lapin Polyclonal anti-KIT Ligand a été validé pour WB et IHC. Il convient pour détecter KIT Ligand dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour KIT Ligand anticorps (N-Term) (ABIN3042665)

Antigène: KIT Ligand (KITLG)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KIT Ligand est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-KIT Ligand anticorps

Épitope: AA 26-44, N-Term

Fonction: Anti-SCF/KITLG Antibody Picoband®

Séquence: DEGICRNRVT NNVKDVTKLV

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-SCF/KITLG Antibody (ABIN3042665). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human SCF.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Geissler, E. N., Liao, M., Brook, J. D., Martin, F. H., Zsebo, K. M., Housman, D. E., Galli, S. J. Stem cell factor (SCF), a novel hematopoietic growth factor and ligand for c-kit tyrosine kinase receptor, maps on human chromosome 12 between 12q14.3 and 12qter. Somat. Cell Molec. Genet. 17: 207-214, 1991. 2. Kent D, Copley M, Benz C, Dykstra B, Bowie M, Eaves C (April 2008). "Regulation of hematopoietic stem cells by the steel factor/KIT signaling pathway". Clin. Cancer Res. 14 (7): 1926-30.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-KIT Ligand (ABIN3042665)

Li, Wu, Miao, Xiong et al.: "Effects of Polysaccharide of Gastrodia Elata Blume and Electro-Acupuncture on Expressions of Brain-Derived Neurotrophic Factor and Stem Cell Factor Protein in Caudate Putamen of Focal Cerebral ..." dans: Medical science monitor basic research, Vol. 22, pp. 175-180, (2017) (PubMed).

Jeng, Jeng, Jeng, Sheen, Li, Lu, Chang: "Tropism of liver epithelial cells toward hepatocellular carcinoma in vitro and in vivo with altering gene expression of cancer stem cells." dans: American journal of surgery, Vol. 215, Issue 4, pp. 735-743, (2017) (PubMed).

Détails sur KIT Ligand

Antigène: KIT Ligand (KITLG)

Autre désignation: KITLG

Sujet:

Synonyms: Kit ligand,Mast cell growth factor,MGF,Stem cell factor,SCF,c-Kit ligand,Soluble KIT ligand,sKITLG,KITLG,MGF, SCF,

Background: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. SCF is localized to the long arm of human chromosome 12, between 12q14.3 and 12qter. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF has been shown to increase the survival of HSCs in vitro and contributes to the self renewal and maintenance of HSCs in-vivo. HSCs at all stages of development express the same levels of the receptor for SCF (c-Kit). The stromal cells that surround HSCs are a component of the stem cell niche, and they release a number of ligands, including SCF.

Sequence Similarities: Belongs to the SCF family.

Poids moléculaire: 18.4 kDa

UniProt: P21583

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway