ITCH anticorps (Middle Region)

N° du produit ABIN3043253

L’anticorps anti-ITCH Polyclonal Lapin est utilisé pour la détection de ITCH dans des échantillons de Humain, Souris, Rat et Singe. Il a été validé pour WB et FACS.

Aperçu rapide pour ITCH anticorps (Middle Region) (ABIN3043253)

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ITCH est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-ITCH anticorps

Épitope: Middle Region

Fonction: Anti-ITCH/AIP4 Antibody

Séquence: AMQQFNQRFI YGNQDLFATS QSKEFDPL

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: different from the related mouse sequence by one amino acid.

Attributs du produit: Anti-ITCH/AIP4 Antibody. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human ITCH/AIP4, different from the related mouse sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur ITCH

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Autre désignation: ITCH

Sujet:

Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: itchy E3 ubiquitin protein ligase

Sequence Similarities: Contains 1 C2 domain.

Poids moléculaire: 103 kDa

ID gène: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events