Dihydrofolate Reductase anticorps (AA 2-187)

N° du produit ABIN3043286

Détail du produit anti-Dihydrofolate Reductase anticorps

Antigène: Dihydrofolate Reductase (DHFR)

Épitope: AA 2-187

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Dihydrofolate Reductase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Dihydrofolate reductase(DHFR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Dihydrofolate reductase(DHFR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: dihydrofolate reductase
Protein Name: Dihydrofolate reductase

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Reacts with: human, mouse, rat

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-Dihydrofolate Reductase (ABIN3043286)

Tian, Zhang, Ge, Xiao, Lu, Fu, Liu, Sun: "MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma." dans: Medical oncology (Northwood, London, England), Vol. 31, Issue 1, pp. 785, (2013) (PubMed).

Détails sur Dihydrofolate Reductase

Antigène: Dihydrofolate Reductase (DHFR)

Autre désignation: DHFR (DHFR Produits)

Synonymes: anticorps DHFR, anticorps F10_orf160, anticorps ECK0049, anticorps JW0047, anticorps tmrA, anticorps cb595, anticorps zgc:86637, anticorps DDBDRAFT_0187132, anticorps DDBDRAFT_0230113, anticorps DDB_0187132, anticorps DDB_0230113, anticorps CG14887, anticorps Dmel\\CG14887, anticorps dhfr, anticorps DHFRP1, anticorps DYR, anticorps 8430436I03Rik, anticorps AA607882, anticorps AI662710, anticorps AW555094, anticorps Dhfr1, anticorps confers resistance to sulfonamides, anticorps dihydrofolate reductase, anticorps dihydrofolate reductase DhfR, anticorps Dihydrofolate reductase, anticorps dhfr, anticorps DHFR, anticorps dhfR, anticorps folA, anticorps dfrA, anticorps LOC707382, anticorps CNJ01940, anticorps DDB_G0286755, anticorps Dhfr, anticorps Mesil_0096, anticorps Arch_0408, anticorps Fbal_3367, anticorps Ilyop_0482, anticorps Lbys_2007, anticorps Riean_0733, anticorps Mrub_1855, anticorps Poras_0766, anticorps Halhy_3552, anticorps Mesop_3614, anticorps LOC609048

Sujet: Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Synonyms: DHFR antibody|DHFRP1 antibody|Dihydrofolate reductase antibody|DYR antibody|DYR_HUMAN antibody|EC 1.5.1.3 antibody

ID gène: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases