Cyclin A anticorps (AA 10-168)

N° du produit ABIN3043481

L’anticorps Lapin Polyclonal anti-Cyclin A a été validé pour WB, IHC, IF, FACS et ICC. Il convient pour détecter Cyclin A dans des échantillons de Humain, Souris et Rat. Il y a 4+ publications disponibles.

Aperçu rapide pour Cyclin A anticorps (AA 10-168) (ABIN3043481)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-Cyclin A anticorps

Épitope: AA 10-168

Fonction: Anti-Cyclin A2/CCNA2 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Cyclin A2/CCNA2 Antibody Picoband® (ABIN3043481). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Cyclin A2 recombinant protein (Position: A10-K168). Human Cyclin A2 shares 74.5% amino acid (aa) sequence identity with mouse Cyclin A2.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Gong D, Ferrell JE (Sep 2010). "The roles of cyclin A2, B1, and B2 in early and late mitotic events". Mol. Biol. Cell 21(18): 3149-61. 2. Yam CH, Fung TK, Poon RY (Aug 2002). "Cyclin A in cell cycle control and cancer". Cell. Mol. Life Sci. 59 (8): 1317-26.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Cyclin A (ABIN3043481)

Zhang, Chang, Zhao, Gao, Wang, Li, Zhang, Zhang, Xu: "The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation." dans: PLoS ONE, Vol. 12, Issue 7, pp. e0180607, (2017) (PubMed).

Zhang, Chang, Li, Zhang, Xu: "The novel protein C3orf43 accelerates hepatocyte proliferation." dans: Cellular & molecular biology letters, Vol. 22, pp. 21, (2017) (PubMed).

Wang, Ge, Chen, Hu, Li, Song, Xu, Ye: "A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21 (Waf1/CIP1) Expression." dans: BioMed research international, Vol. 2015, pp. 304753, (2016) (PubMed).

Chen, Zhang, Liang, Zhou, Ding, Yang, Liu, Wu, Man, Zhang, Chen: "Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells." dans: Cell communication and signaling : CCS, Vol. 12, pp. 18, (2014) (PubMed).

Détails sur Cyclin A

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: CCNA2

Sujet:

Synonyms: Cyclin-A2,Cyclin-A,CCNA2,CCN1, CCNA,

Tissue Specificity: Ubiquitous.

Background: Cyclin A2, known as CCNA2, is mapped to 4q27. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. And Cyclin A2 is synthesized at the onset of S phase and localizes to the nucleus, where the cyclin A2-CDK2 complex is implicated in the initiation and progression of DNA synthesis. Phosphorylation of CDC6 and MCM4 by the cyclin A2-CDK2 complex prevents re-replication of DNA during the cell cycle.

Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.

Poids moléculaire: 55 kDa

ID gène: 890

UniProt: P20248

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA