Survivin anticorps (AA 1-140)

N° du produit ABIN3043511

L’anticorps Lapin Polyclonal anti-Survivin a été validé pour WB, IHC et FACS. Il convient pour détecter Survivin dans des échantillons de Souris. Il y a 9+ publications disponibles.

Aperçu rapide pour Survivin anticorps (AA 1-140) (ABIN3043511)

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Survivin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-Survivin anticorps

Épitope: AA 1-140

Fonction: Anti-Survivin/BIRC5 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Survivin/BIRC5 Antibody (ABIN3043511). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mous
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
1. Ambrosini, G., Adida, C., Altieri, D. C.A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma.Nature Med. 3: 917-921, 1997. 2. Chantalat, L., Skoufias, D. A., Kleman, J.-P., Jung, B., Dideberg, O., Margolis, R. L.Crystal structure of human survivin reveals a bow tie-shaped dimer with two unusual alpha-helical extensions.Molec. Cell 6: 183-189, 2000.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Survivin (ABIN3043511)

Gu, Ren, Wang, Qu, Zhang: "Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 11, pp. 14034-9, (2016) (PubMed).

Zhang, Wang, Yang, Pan, Li, Yuan: "Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing." dans: Oncology letters, Vol. 11, Issue 5, pp. 3265-3272, (2016) (PubMed).

Li, Zhao, Lu, Li, Yang, Wu, Liu: "Notch-1 signaling promotes the malignant features of human breast cancer through NF-?B activation." dans: PLoS ONE, Vol. 9, Issue 4, pp. e95912, (2014) (PubMed).

Zhang, Lu, Hou, Hu, Wang: "The effects of STAT3 and Survivin silencing on the growth of human bladder carcinoma cells." dans: Tumour biology, Vol. 35, Issue 6, pp. 5401-7, (2014) (PubMed).

Chen, Xie, Zhao, Wang, Bai, Yin, Jiang, Xie, Jia, Huang et al.: "Ampelopsin induces apoptosis by regulating multiple c?Myc/S?phase kinase?associated protein 2/F?box and WD repeat?containing protein 7/histone deacetylase 2 pathways in human lung adenocarcinoma ..." dans: Molecular medicine reports, Vol. 11, Issue 1, pp. 105-12, (2014) (PubMed).

Hao, Huang, Liao, Zhu, Liu, Hao, Liu, Zhang, Feng, Ning, Li, Li: "The inhibition of resveratrol to human skin squamous cell carcinoma A431 xenografts in nude mice." dans: Fitoterapia, Vol. 86, pp. 84-91, (2013) (PubMed).

Li, Zhang, Fang, Yan, Zhao, Feng, Ma, Ye: "Aspirin overcomes Navitoclax-resistance in hepatocellular carcinoma cells through suppression of Mcl-1." dans: Biochemical and biophysical research communications, Vol. 434, Issue 4, pp. 809-14, (2013) (PubMed).

Cai, Liu, Zhao, Wan, Hu, Zhou, Chen: "Expressions of survivin and vascular endothelial growth factor in a Murine model of proliferative retinopathy." dans: International journal of ophthalmology, Vol. 5, Issue 3, pp. 293-6, (2012) (PubMed).

Shan, Zhang, Geng, Zhou, Chen, Nie, Guo, Li, Liu, Guo, Wei, Chen: "Expression of survivin and human telomerase reverse transcriptase in extramammary Paget's disease." dans: Journal of cutaneous pathology, Vol. 37, Issue 6, pp. 635-40, (2010) (PubMed).

Détails sur Survivin

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Autre désignation: BIRC5

Sujet:

Synonyms: Baculoviral IAP repeat-containing protein 5,Apoptosis inhibitor 4,Apoptosis inhibitor survivin,TIAP,Birc5,Api4, Iap4,

Tissue Specificity: Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions. .

Background: Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.

Sequence Similarities: Belongs to the IAP family.

Poids moléculaire: 16 kDa

ID gène: 11799

UniProt: O70201

Pathways: Apoptose, Cycle Cellulaire, Nuclear Hormone Receptor Binding