POLB anticorps (AA 122-335)

N° du produit ABIN3043545

Détail du produit anti-POLB anticorps

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Épitope: AA 122-335

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLB est non-conjugé

Application: Western Blotting (WB)

Fonction: Rabbit IgG polyclonal antibody for DNA polymerase beta(POLB) detection. Tested with WB in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for DNA polymerase beta(POLB) detection. Tested with WB in Human.
Gene Name: polymerase (DNA directed), beta
Protein Name: DNA polymerase beta

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur POLB

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Autre désignation: POLB (POLB Produits)

Synonymes: anticorps wu:fa97a05, anticorps zgc:109924, anticorps POLB, anticorps DDBDRAFT_0186152, anticorps DDBDRAFT_0215784, anticorps DDBDRAFT_0232376, anticorps DDB_0186152, anticorps DDB_0215784, anticorps DDB_0232376, anticorps A430088C08Rik, anticorps polymerase (DNA directed), beta, anticorps DNA polymerase beta, anticorps DNA polymerase X family protein, anticorps polymerase (DNA directed), beta S homeolog, anticorps polb, anticorps POLB, anticorps polB, anticorps polb.S, anticorps Polb

Sujet: Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

Synonyms: DNA directed DNA polymerase beta antibody|DNA pol beta antibody|DNA polymerase beta antibody|DNA polymerase beta subunit antibody| DPOLB_HUMAN antibody|MGC125976 antibody|Pol B antibody|Pol beta antibody|POLB antibody|Polymerase (DNA directed) beta antibody

ID gène: 5423

UniProt: P06746

Pathways: Réparation de l'ADN