NLRP3 anticorps (AA 21-194)

N° du produit ABIN3043571

L’anticorps Lapin Polyclonal anti-NLRP3 a été validé pour WB. Il convient pour détecter NLRP3 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour NLRP3 anticorps (AA 21-194) (ABIN3043571)

Antigène: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NLRP3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-NLRP3 anticorps

Épitope: AA 21-194

Fonction: Anti-CIAS1/NALP3/NLRP3 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® (ABIN3043571). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
1. Agostini, L., Martinon, F., Burns, K., McDermott, M. F., Hawkins, P. N., Tschopp, J. NALP3 forms an IL-1-beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. 2. Allen, I. C., Scull, M. A., Moore, C. B., Holl, E. K., McElvania-TeKippe, E., Taxman, D. J., Guthrie, E. H., Pickles, R. J., Ting, J. P.-Y. The NLRP3 inflammasome mediates in vivo innate immunity to influenza A virus through recognition of viral RNA. Immunity 30: 556-565, 2009. 3. Hoffman HM, Wright FA, Broide DH, Wanderer AA, Kolodner RD (Jun 2000). "Identification of a locus on chromosome 1q44 for familial cold urticaria". Am J Hum Genet 66 (5): 1693-8.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-NLRP3 (ABIN3043571)

Zhang, Li, Xu, Xiang, Ma: "Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." dans: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).

Xue, Wang, Du, Zhu: "Maternal obesity induces gut inflammation and impairs gut epithelial barrier function in nonobese diabetic mice." dans: The Journal of nutritional biochemistry, Vol. 25, Issue 7, pp. 758-64, (2014) (PubMed).

Wang, Yang, Liang, Zhu, Du: "Grape seed extract prevents skeletal muscle wasting in interleukin 10 knockout mice." dans: BMC complementary and alternative medicine, Vol. 14, pp. 162, (2014) (PubMed).

Détails sur NLRP3

Antigène: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Autre désignation: NLRP3

Sujet:

Synonyms: NACHT, LRR and PYD domains-containing protein 3,Angiotensin/vasopressin receptor AII/AVP-like,Caterpiller protein 1.1,CLR1.1,Cold autoinflammatory syndrome 1 protein,Cryopyrin,PYRIN-containing APAF1-like protein 1,NLRP3,C1orf7, CIAS1, NALP3, PYPAF1,

Tissue Specificity: Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. .

Background: NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. It is mapped to 1q44. The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, it has been showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Sequence Similarities: Belongs to the NLRP family.

Poids moléculaire: 118 kDa

ID gène: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome