PKLR anticorps (C-Term)

N° du produit ABIN3043607

L’anticorps Lapin Polyclonal anti-PKLR a été validé pour WB et IHC. Il convient pour détecter PKLR dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PKLR anticorps (C-Term) (ABIN3043607)

Antigène: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKLR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-PKLR anticorps

Épitope: AA 522-552, C-Term

Fonction: Anti-PKLR Antibody Picoband®

Séquence: EAIWADDVDR RVQFGIESGK LRGFLRVGDL V

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PKLR Antibody Picoband® (ABIN3043607). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR, different from the related mouse sequence by one amino acid, and identical to the rat sequence.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: PKLR pyruvate kinase, liver and RBC". 2. Tani K, Fujii H, Tsutsumi H, Sukegawa J, Toyoshima K, Yoshida MC, Noguchi T, Tanaka T, Miwa S (Apr 1987). "Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment". Biochem Biophys Res Commun 143 (2): 431-8.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PKLR

Antigène: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Autre désignation: PKLR

Sujet:

Synonyms: Pyruvate kinase PKLR,2.7.1.40,Pyruvate kinase 1,Pyruvate kinase isozymes L/R,R-type/L-type pyruvate kinase,Red cell/liver pyruvate kinase,PKLR,PK1, PKL,

Tissue Specificity: Ubiquitous, expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.

Background: Pyruvate kinase isozymes R/L is an enzyme that in humans is encoded by the PKLR gene. It is mapped to 1q21. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the pyruvate kinase family.

Poids moléculaire: 62 kDa

ID gène: 5313

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process