Glutaredoxin 2 anticorps (Middle Region)

N° du produit ABIN3044103

Cet anticorps anti-Glutaredoxin 2 est un anticorps Lapin Polyclonal détectant Glutaredoxin 2 dans WB et IHC. Adapté pour Humain, Rat et Souris.

Aperçu rapide pour Glutaredoxin 2 anticorps (Middle Region) (ABIN3044103)

Antigène: Glutaredoxin 2 (GRX2)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Glutaredoxin 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Glutaredoxin 2 anticorps

Épitope: AA 103-119, Middle Region

Fonction: Anti-Glutaredoxin 2/GLRX2 Antibody Picoband®

Séquence: EYGNQFQDAL YKMTGER

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Glutaredoxin 2/GLRX2 Antibody (ABIN3044103). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human Glutaredoxin 2, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
1. Gladyshev, V. N., Liu, A., Novoselov, S. V., Krysan, K., Sun, Q.-A., Kryukov, V. M., Kryukov, G. V., Lou, M. F.Identification and characterization of a new mammalian glutaredoxin (thioltransferase), Grx2.J. Biol. Chem. 276: 30374-30380, 2001. 2. Lilig, C. H., Berndt, C., Vergnolle, O., Lonn, M. E., Hudemann, C., Bill, E., Holmgren, A.Characterization of human glutaredoxin 2 as iron-sulfur protein: a possible role as redox sensor.Proc. Nat. Acad. Sci. 102: 8168-8173, 2005. 3. Lundberg, M., Johansson, C., Chandra, J., Enoksson, M., Jacobsson, G., Ljung, J., Johansson, M., Holmgren, A.Cloning and expression of a novel human glutaredoxin (Grx2) with mitochondrial and nuclear isoforms.J. Biol. Chem. 276: 26269-26275, 2001.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.01 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur Glutaredoxin 2

Antigène: Glutaredoxin 2 (GRX2)

Autre désignation: GLRX2

Sujet:

Synonyms: Glutaredoxin-2, mitochondrial,GLRX2,GRX2,CGI-133,

Tissue Specificity: Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes. .

Background: GLRX2 (Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins (e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al. (2001) and Gladyshev et al. (2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al. (2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon (exon 1B) located upstream from the first exon used by the GLRX2A transcript (exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al. (2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.

Sequence Similarities: Belongs to the glutaredoxin family.

Poids moléculaire: 18 kDa

Pathways: Cell RedoxHomeostasis