GLI3 anticorps (N-Term)

N° du produit ABIN3044156

Détail du produit anti-GLI3 anticorps

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Épitope: AA 40-57, N-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI3 est non-conjugé

Application: Western Blotting (WB)

Fonction: Rabbit IgG polyclonal antibody for Transcriptional activator GLI3(GLI3) detection. Tested with WB in Human,Mouse,Rat.

Séquence: SNEDESPGQT YHRERRNA

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Transcriptional activator GLI3(GLI3) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: GLI family zinc finger 3
Protein Name: Transcriptional activator GLI3

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3(40-57aa SNEDESPGQTYHRERRNA), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-GLI3 (ABIN3044156)

Kumari, Chaurasia, Kumar, Dash: "Anti-apoptotic role of sonic hedgehog on blood platelets." dans: Thrombosis research, Vol. 134, Issue 6, pp. 1311-5, (2014) (PubMed).

Détails sur GLI3

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: GLI3 (GLI3 Produits)

Sujet: GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product(relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus(ci) gene product(Ci), which regulates the Patched(pct), gooseberry(gsb), and decapentaplegic(dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

Synonyms: ACLS antibody|DNA binding protein antibody|GCPS antibody|Gli 3 antibody|GLI family zinc finger 3 antibody|GLI Kruppel family member GLI 3 antibody|GLI Kruppel family member GLI3(Greig cephalopolysyndactyly syndrome) antibody|GLI Kruppel family member GLI3 antibody|GLI3 antibody|GLI3 C-terminally truncated form antibody|GLI3 form of 190 kDa antibody|GLI3 form of 83 kDa antibody|GLI3 full length protein antibody|GLI3-190 antibody|GLI3-83 antibody|GLI3_HUMAN antibody|GLI3FL antibody|Glioma associated oncogene family zinc finger 3 antibody|Oncogene GLI3 antibody|PAP A antibody|PAPA 1 antibody|PAPA antibody|PAPA1 antibody|PAPB antibody|PHS antibody|PPD IV antibody|PPDIV antibody|Transcriptional activator GLI3 antibody|Transcriptional repressor GLI3R antibody|Zinc finger protein GLI 3 antibody|Zinc finger protein GLI3 antibody

UniProt: P10071

Pathways: Signalisation Hedgehog