Adenosine A2a Receptor anticorps (3rd Intracellular Domain)

N° du produit ABIN305729

L’anticorps Souris Monoclonal anti-Adenosine A2a Receptor a été validé pour WB, IHC, FACS, IHC (p) et IHC (pfa). Il convient pour détecter Adenosine A2a Receptor dans des échantillons de Humain, Souris, Rat, Chien, Lapin et Cobaye.

Aperçu rapide pour Adenosine A2a Receptor anticorps (3rd Intracellular Domain) (ABIN305729)

Antigène: Adenosine A2a Receptor (ADORA2A)

Reactivité: Humain, Souris, Rat, Chien, Lapin, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Adenosine A2a Receptor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Clone: 7F6-G5-A2-7F6

Détail du produit anti-Adenosine A2a Receptor anticorps

Épitope: 3rd Intracellular Domain

Marque: IHC-plus™

Specificité: Recognizes human adenosine receptor A2A at ~43kD.

Purification: Protein G purified

Immunogène: 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, IHC, IHC-P (5 μg/mL), IHC-Pfa (1 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Adenosine A2a Receptor

Antigène: Adenosine A2a Receptor (ADORA2A)

Autre désignation: ADORA2A/Adenosine A2A Receptor

Sujet: Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

ID gène: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints