Caspase 3 anticorps

N° du produit ABIN305732

L’anticorps anti-Caspase 3 Monoclonal Souris est utilisé pour la détection de Caspase 3 dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC et IHC (p).

Aperçu rapide pour Caspase 3 anticorps (ABIN305732)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 31A1067 

Détail du produit anti-Caspase 3 anticorps

Specificité: Detects both pro Caspase-3 (~32 kD) and the large subunit of the active/cleaved form (~14-21 kD) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this in Western blot applications.

Purification: Protein G purified

Immunogène: Full length recombinant human Caspase-3 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (2 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: CASP3 / Caspase 3

Sujet: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

ID gène: 836

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation