ERBB3 anticorps (AA 1330-1342)

N° du produit ABIN305733

Cet anticorps anti-ERBB3 Polyclonal Chèvre (ABIN305733) détecte spécifiquement ERBB3 dans WB, IHC, ELISA et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Rat, Singe, Cheval, Porc, Roussette (Chauve-souris) et Hamster.

Aperçu rapide pour ERBB3 anticorps (AA 1330-1342) (ABIN305733)

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivité: Humain, Rat, Singe, Cheval, Porc, Roussette (Chauve-souris), Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ERBB3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ERBB3 anticorps

Épitope: AA 1330-1342

Specificité: Human ERBB3. This antibody is expected to recognise isoform 1 (NP_001973.2) only.

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Hamster, Panda, Horse, Pig (100%) Mouse, Bovine (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-HSRLFPKANAQRT from the C-terminus of human ERBB3 (NP_001973.2). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Hamster, Panda, Horse, Pig (100%), Mouse, Bovine (92%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ERBB3

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Autre désignation: HER3 / ERBB3

Sujet: Name/Gene ID: ERBB3
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3

ID gène: 2065

NCBI Accession: NP_001973

UniProt: P21860

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway