Cyclin E1 anticorps (full length)

N° du produit ABIN306564

Cet anticorps anti-Cyclin E1 Monoclonal Souris (Clone 168) (ABIN306564) détecte spécifiquement Cyclin E1 dans WB et IP. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Cyclin E1 anticorps (full length) (ABIN306564)

Antigène: Cyclin E1 (CCNE1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin E1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 168

Détail du produit anti-Cyclin E1 anticorps

Épitope: full length

Fonction: Mouse anti Human cyclin E

Attributs du produit: Human cyclin E was originally identified by complementation of a triple cln deletion in Saccharomyces cerevisiae. Following the identification of human cyclin E protein, researchers found that breast cancers and some other solid tumors exhibited both quantitative and qualitative alterations in cyclin E protein production. In particular, the alterations in cyclin E expression in breast cancer correlated with increasing stage and grade of the tumor. These results suggested that cyclin E might be useful as a prognostic marker. More recently, Geng et al generated a mouse strain in which the coding sequences for cyclin D1 was deleted and replaced with those of human cyclin E. Replacement of cyclin D1 with cyclin E rescued all phenotypic manifestations seen in the cyclin D1 deficient mice and restored normal development to cyclin D1-dependent tissues. Based on the results of these studies, it appears that cyclin E is the major downstream target of cyclin D1. In addition to functioning downstream of cyclin D1, recent studies with Xenopus egg extracts demonstrated that cyclin E localizes to the centrosome. In these studies CDK2-cyclin E activity was shown to be required for centrosome duplication during S phase. Taken together, these data suggest a mechanism that coordinates centrosome reproduction with cycles of DNA synthesis and mitosis.

Purification: Protein A/G Chromatography

Immunogène: Recombinant full-length human cyclin E protein

Isotype: IgG2a

Information d'application

Indications d'application: Antibody detects human cyclin E protein by Western blot. Detects a 50 kDa band. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur Cyclin E1

Antigène: Cyclin E1 (CCNE1)

Autre désignation: cyclin E

Sujet: G1/S-specific cyclin E1, CCNE1

UniProt: P24864

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases