Caspase 3 anticorps

N° du produit ABIN306792

Cet anticorps anti-Caspase 3 Monoclonal Souris (Clone AM1 4) (ABIN306792) détecte spécifiquement Caspase 3 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Caspase 3 anticorps (ABIN306792)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 3 est non-conjugé

Application: Western Blotting (WB)

Clone: AM1 4

Détail du produit anti-Caspase 3 anticorps

Fonction: Mouse anti Human Caspase-3

Réactivité croisée: Humain

Attributs du produit: Caspase-3 along with caspase 7 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three varient forms. Caspase-3-like activities are required for Fas-mediated apoptosis. However, the role of caspase-1 and caspase-3 in mediating Fas-induced cell death is not clear. Although wild-type, caspase-1(-/-), and caspase-3(-/-) hepatocytes were killed at a similar rate when cocultured with FasL expressing NIH 3T3 cells, caspase-3(-/-) hepatocytes displayed drastically different morphological changes as well as significantly delayed DNA fragmentation. For both wild-type and caspase-1 (-/-) apoptotic hepatocytes, typical apoptotic features such as cytoplasmic blebbing and nuclear fragmentation are seen within 6 hr, but neither event was observed for caspase-3(-/-) hepatocytes. In thymocytes apoptotic caspase-3 (-/-) thymocytes exhibit similar abnormal morphological changes and delayed DNA fragmentation observed in hepatocytes. Cleavage of various caspase substrates implicates apoptotic events, including gelsolin, fodrin, laminB, and DFF45/ICAD are delayed or absent. The altered cleavage of these key substrates is likely responsible for the aberrant apoptosis observed in both hepatocytes and thymocytes deficient in caspase-3.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human Caspase-3 protein and mouse myeloma cells.

Isotype: IgG

Information d'application

Indications d'application: Detects human Caspase-3 by Western blot. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: Caspase-3

Sujet: Cysteine-requiring Aspartate Protease-3

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation