HSP27 anticorps

N° du produit ABIN306830

L’anticorps Souris Monoclonal anti-HSP27 a été validé pour WB, ICC, ELISA, IP, EIA, IHC (fro) et IHC (p). Il convient pour détecter HSP27 dans des échantillons de Humain.

Aperçu rapide pour HSP27 anticorps (ABIN306830)

Antigène: HSP27 (Heat Shock Protein 27 (HSP27))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: G3-1

Détail du produit anti-HSP27 anticorps

Réactivité croisée: Humain

Attributs du produit: HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosphilia HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with partially purified HSP27 derived from MCF-7 cytosol and mouse myeloma cells.

Isotype: IgG1

Information d'application

Indications d'application: This antibody can be used for immunohistochemistry and immunocytochemistry on frozen and paraffin-embedded tissue sections as well as for ELISA, immunoprecipitation and Western blot. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur HSP27

Antigène: HSP27 (Heat Shock Protein 27 (HSP27))

Autre désignation: Heat Shock Protein 27

UniProt: P04792

Pathways: VEGF Signaling