MITF anticorps (N-Term)

N° du produit ABIN306846

Cet anticorps anti-MITF est un anticorps Souris Monoclonal détectant MITF dans WB, IHC (p), IP, GS et IHC (fro). Adapté pour Humain.

Aperçu rapide pour MITF anticorps (N-Term) (ABIN306846)

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MITF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Gel Shift (GS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: C5

Détail du produit anti-MITF anticorps

Épitope: N-Term

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: MiTF,There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. MiTF is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteoporotic rat strain harbors a large genomic deletion encompassing the 3 prime half of MiTF including most of the b-HLH-ZIP region. Osteoclasts from these animals lack MiTF protein in contrast to wild-type rat, mouse, and human osteoclasts.

Purification: Protein A/G Chromatography

Immunogène: N-terminal fragment of human microphthalmia protein.,Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.

Isotype: IgG1

Information d'application

Indications d'application: This antibody can be used for gel supershift assays, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/mL) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, antibody recognizes a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.

Restrictions: For Research Use only

Stockage

Buffer: Purified monoclonal antibody clone C5 in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur MITF

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Autre désignation: Microphthalmia Transcription Factor (MiTF)

UniProt: O75030

Pathways: Chromatin Binding