SIAH1 anticorps

N° du produit ABIN307027

Détail du produit anti-SIAH1 anticorps

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIAH1 est non-conjugé

Application: Western Blotting (WB)

Réactivité croisée: Humain

Attributs du produit: SIAH1 (seven in absentia homolog 1), E3 Ubiquitin Ligase,Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1. Both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity.

Purification: Ammonium Sulfate Precipitation

Immunogène: Synthetic peptide derived from the human SIAH-1 protein.

Isotype: IgG

Information d'application

Indications d'application: Antibody can be used for Western blotting (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur SIAH1

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Autre désignation: SIAH1 (SIAH1 Produits)

Synonymes: anticorps SIAH, anticorps Siah1, anticorps Siah1a, anticorps id:ibd2635, anticorps zgc:56026, anticorps SIAH1A, anticorps siah, anticorps E3 ubiquitin-protein ligase Siah1, anticorps E3 ubiquitin-protein ligase siah-1, anticorps siah E3 ubiquitin protein ligase 1, anticorps siah E3 ubiquitin protein ligase 1 L homeolog, anticorps Tsp_03055, anticorps siah-1, anticorps Siah1, anticorps siah1, anticorps SIAH1, anticorps siah1.L

UniProt: Q8IUQ4