Clusterin anticorps

N° du produit ABIN307177

Cet anticorps Souris Monoclonal détecte spécifiquement Clusterin dans WB, IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Clusterin anticorps (ABIN307177)

Antigène: Clusterin (CLU)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Clusterin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Clusterin anticorps

Specificité: Recognizes human clusterin, alpha at 40kD, and precursor protein.

Purification: Protein G purified

Immunogène: Native clusterin purified from human serum.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 40 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Détails sur Clusterin

Antigène: Clusterin (CLU)

Autre désignation: CLU / Clusterin

Sujet: Name/Gene ID: CLU

Synonyms: CLU, APO-J, Apolipoprotein J, CLU1, Clusterin, Complement lysis inhibitor, CLI, AAG4, Aging-associated protein 4, Ku70-binding protein 1, KUB1, SGP2, APOJ, SGP-2, Sulfated glycoprotein 2, CLU2, Complement cytolysis inhibitor, NA1/NA2, SP-40

ID gène: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling