Transglutaminase 2 anticorps

N° du produit ABIN307194

Cet anticorps anti-Transglutaminase 2 Polyclonal Lapin (ABIN307194) détecte spécifiquement Transglutaminase 2 dans WB, IHC, IP et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Transglutaminase 2 anticorps (ABIN307194)

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Transglutaminase 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Transglutaminase 2 anticorps

Specificité: Recognizes human Transglutaminase II at ~77-85kD.

Purification: Protein A purified

Immunogène: Recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), IP, WB (5 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation (Native verified): 10 μg/mg protein lysate. Use Protein A. Western Blot: 5-10 μg/mL for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Détails sur Transglutaminase 2

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Autre désignation: TGM2 / Transglutaminase 2

Sujet: Name/Gene ID: TGM2

Synonyms: TGM2, G-ALPHA-h, GNAH, TG(C), Transglutaminase 2, Transglutaminase C, TG2, TGase H, TGase-2, TGase-H, Transglutaminase H, TGase C, TGC, TTG, C polypeptide, Tgase II, Tissue transglutaminase, Transglutaminase-2

ID gène: 7052

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling