CD80 anticorps

N° du produit ABIN316880

Cet anticorps Rat Monoclonal détecte spécifiquement CD80 dans FACS et IHC (fro). Il présente une réactivité envers Souris.

Aperçu rapide pour CD80 anticorps (ABIN316880)

Antigène: CD80

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD80 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RM80

Détail du produit anti-CD80 anticorps

Specificité: This antibody recognises CD80 (B7-1), a 60 kDa cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells. Expression may be significantly increased upon B lymphocytes by LPS and by IL-4.

Attributs du produit: Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogène: BCL1 cells expressing CD80

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: 1/10 - 1/25, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL. Immunohistochemistry on frozen sections. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline, pH 7.4 containing 0,09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD80

Antigène: CD80

Autre désignation: CD80

Sujet: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

ID gène: 12519

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints