VAMP2 anticorps (N-Term)

N° du produit ABIN317061

Cet anticorps anti-VAMP2 est un anticorps Souris Monoclonal détectant VAMP2 dans WB, IHC (p) et EIA. Adapté pour Humain et Rat.

Aperçu rapide pour VAMP2 anticorps (N-Term) (ABIN317061)

Antigène: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VAMP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 3-00E-005

Détail du produit anti-VAMP2 anticorps

Épitope: AA 1-89, N-Term

Specificité: This antibody recognizes specifically Synaptobrevin 2, but it also shows a low affinity to Synaptobrevin 1. Detects a band of approximately 14 kDa in rat brain lysates.

Attributs du produit: Synonyms: VAMP2, Vesicle-associated membrane protein 2, SYB2

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogène: Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western blot (1/2,000-1/3,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated foruse in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur VAMP2

Antigène: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Autre désignation: VAMP-2 / Synaptobrevin-2

Sujet: Synaptobrevin 2 (Syb2) also known as vesicle associated membrane protein-2 (VAMP2) is a membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. Synaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.Synonyms: SYB2, VAMP2, Vesicle-associated membrane protein 2

ID gène: 6844

UniProt: P63027

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport