Interferon gamma anticorps

N° du produit ABIN317387

L’anticorps Souris Monoclonal anti-Interferon gamma a été validé pour WB, FACS et IHC (p). Il convient pour détecter Interferon gamma dans des échantillons de Boeuf (Vache).

Aperçu rapide pour Interferon gamma anticorps (ABIN317387)

Antigène: Interferon gamma (IFNG)

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Interferon gamma est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7B6

Détail du produit anti-Interferon gamma anticorps

Specificité: This antibody recognises both native and recombinant IFN gamma.

Attributs du produit: Synonyms: IFN-gamma, gamma IFN

Purification: Purified IgG prepared by affinity chromatography on Protein G

Isotype: IgG1

Information d'application

Indications d'application: Western blot. Flow cytometry: 1/500 - 1/2000, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL (Membrane permeabilisation is required for this application, we recommendthe use of Leukoperm). Immunohistochemistry on paraffin sections (requires antigen retrieval using heattreatment prior to staining, Sodium citrate buffer pH 6.0 is recommended). Does not work in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Interferon gamma

Antigène: Interferon gamma (IFNG)

Autre désignation: IFNG / Interferon gamma

Sujet: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

ID gène: 281237

UniProt: P07353

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy