DNMT3B anticorps

N° du produit ABIN317617

Cet anticorps Lapin Polyclonal détecte spécifiquement DNMT3B dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour DNMT3B anticorps (ABIN317617)

Antigène: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT3B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DNMT3B anticorps

Specificité: This antibody detects endogenous levels of Dnmt3b protein.(region surrounding His7)

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0,05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur DNMT3B

Antigène: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Autre désignation: DNMT3B

Sujet: Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.Synonyms: DNA (cytosine-5)-methyltransferase 3B, DNA MTase HsaIIIB, DNA methyltransferase HsaIIIB, M.HsaIIIB

ID gène: 1789

NCBI Accession: NP_008823

UniProt: Q9UBC3