PAK4 anticorps

N° du produit ABIN317882

L’anticorps anti-PAK4 Polyclonal Lapin est utilisé pour la détection de PAK4 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour PAK4 anticorps (ABIN317882)

Antigène: PAK4 (P21-Activated Kinase 4 (PAK4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAK4 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PAK4 anticorps

Specificité: PAK4/5/6 antibody detects endogenous levels of PAK4/5/6 protein.(region surrounding Val469)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % Sodium Azide as preservative, approx. pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PAK4

Antigène: PAK4 (P21-Activated Kinase 4 (PAK4))

Autre désignation: PAK4

Sujet: Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.Synonyms: KIAA1142, KIAA1264, PAK 4, PAK-4, PAK-5, PAK-5, PAK-6, PAK-7, PAK5, Serine/threonine-protein kinase PAK 4, Serine/threonine-protein kinase PAK 6, Serine/threonine-protein kinase PAK 7, p21-activated kinase 4, p21-activated kinase 5, p21-activated kinase 6, p21-activated kinase 7

Poids moléculaire: approx. 80 kDa

ID gène: 10298

NCBI Accession: NP_001014831

UniProt: O96013

Pathways: Signalisation RTK