Aurora Kinase B anticorps (AA 227-238)

N° du produit ABIN318827

L’anticorps anti-Aurora Kinase B Polyclonal Lapin est utilisé pour la détection de Aurora Kinase B dans des échantillons de Humain, Souris, Rat, Singe, Boeuf (Vache), Porc et Chien. Il a été validé pour WB et EIA.

Aperçu rapide pour Aurora Kinase B anticorps (AA 227-238) (ABIN318827)

Antigène: Aurora Kinase B (AURKB)

Reactivité: Humain, Souris, Rat, Singe, Boeuf (Vache), Porc, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Aurora Kinase B est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Aurora Kinase B anticorps

Épitope: AA 227-238

Specificité: This antibody detects Aurora-B kinase (AurB).

Réactivité croisée (Details): Species reactivity (expected):Rat, Bovine, Mouse, Chimpanzee, Dog, Pig.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogène: Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1: 2000 - 1: 10000. Western Blot: 1: 250 - 1: 1000, detects a band of approximately 39 kDa in HeLa cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Aurora Kinase B

Antigène: Aurora Kinase B (AURKB)

Autre désignation: Aurora Kinase B

Sujet: Aurora-B kinase is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localisation, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12

ID gène: 9212

NCBI Accession: NP_004208

UniProt: Q96GD4

Pathways: TCR Signaling, Cycle Cellulaire, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades