CD3 anticorps (Cytoplasmic Domain) (FITC)

N° du produit ABIN320000

L’anticorps Rat Monoclonal anti-CD3 a été validé pour FACS. Il convient pour détecter CD3 dans des échantillons de Humain, Poulet, Singe, Porc, Chien, Cheval et Boeuf (Vache). Il y a 1 publication disponible.

Aperçu rapide pour CD3 anticorps (Cytoplasmic Domain) (FITC) (ABIN320000)

Antigène: CD3

Reactivité: Humain, Poulet, Singe, Porc, Chien, Cheval, Boeuf (Vache)

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: CD3-12

Détail du produit anti-CD3 anticorps (FITC)

Épitope: Cytoplasmic Domain

Séquence: Glu-Arg-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys

Specificité: This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. Cross reacts with Bovine, Dog, Horse, Rhesus Monkey, Pig, Mouse and Chicken.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogène: Synthetic peptide sequence derived from cytoplasmic epitope of CD3.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/5-1/10 diluted antibody to label 10^6 cells in 100 μL. Membrane permeabilisation is required. We recommend use of Leucoperm for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-CD3 (FITC) (ABIN320000)

Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

ID gène: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway