FAS anticorps (Extracellular Domain) (PE)

N° du produit ABIN323507

L’anticorps Souris Monoclonal anti-FAS a été validé pour WB, FACS et ELISA. Il convient pour détecter FAS dans des échantillons de Cat.

Aperçu rapide pour FAS anticorps (Extracellular Domain) (PE) (ABIN323507)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est conjugé à/à la PE

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Détail du produit anti-FAS anticorps (PE)

Épitope: Extracellular Domain

Specificité: Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas.

Purification: Protein G purified

Immunogène: NS0-derived recombinant feline rfeFas extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Cat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95 / FAS

Sujet: Name/Gene ID: FAS
Family: TNF Receptor

Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95

ID gène: 355

UniProt: P25445

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity