FAS anticorps (Extracellular Domain) (PE)
-
- Antigène Voir toutes FAS Anticorps
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
-
Épitope
- Extracellular Domain
-
Reactivité
- Cat
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp FAS est conjugé à/à la PE
-
Application
- Western Blotting (WB), Flow Cytometry (FACS), ELISA
- Specificité
- Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas.
- Purification
- Protein G purified
- Immunogène
-
NS0-derived recombinant feline rfeFas extracellular domain.
Type of Immunogen: Recombinant protein - Isotype
- IgG1
- Top Product
- Discover our top product FAS Anticorps primaire
-
-
- Indications d'application
-
Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Commentaires
-
Target Species of Antibody: Cat
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
-
- Antigène
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- Autre désignation
- CD95 / FAS (FAS Produits)
- Synonymes
- anticorps ALPS1A, anticorps APO-1, anticorps APT1, anticorps CD95, anticorps FAS1, anticorps FASTM, anticorps TNFRSF6, anticorps AI196731, anticorps APO1, anticorps TNFR6, anticorps Tnfrsf6, anticorps lpr, anticorps Fas cell surface death receptor, anticorps Fas (TNF receptor superfamily member 6), anticorps FAS, anticorps Fas, anticorps fas
- Sujet
-
Name/Gene ID: FAS
Family: TNF Receptor
Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95 - ID gène
- 355
- UniProt
- P25445
- Pathways
- Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
-