PODXL anticorps (Extracellular Domain)

N° du produit ABIN325654

Cet anticorps Souris Monoclonal détecte spécifiquement PODXL dans WB, FACS et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour PODXL anticorps (Extracellular Domain) (ABIN325654)

Antigène: PODXL (Podocalyxin-Like (PODXL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PODXL est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-PODXL anticorps

Épitope: Extracellular Domain

Specificité: Recognizes human Podocalyxin. In Western Blots, this antibody does not cross-react with rmPodocalyxin or rhEndoglycan.

Purification: Protein G purified

Immunogène: NS0-derived rhPodocalyxin extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, ICC (10 μg/mL), WB

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Immunocytochemistry: 10 μg/mL using fixed cells. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Antibody binding may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: t 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhPodocalyxin is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur PODXL

Antigène: PODXL (Podocalyxin-Like (PODXL))

Autre désignation: PODXL / Podocalyxin

Sujet: Name/Gene ID: PODXL

Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81

ID gène: 5420

UniProt: O00592

Pathways: Tube Formation