CCL13 anticorps

N° du produit ABIN328356

Cet anticorps anti-CCL13 est un anticorps Souris Monoclonal détectant CCL13 dans ELISA, Neut, Func et FACS. Adapté pour Humain.

Aperçu rapide pour CCL13 anticorps (ABIN328356)

Antigène: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CCL13 est non-conjugé

Application: ELISA, Neutralization (Neut), Functional Studies (Func), Flow Cytometry (FACS)

Clone: 73506

Détail du produit anti-CCL13 anticorps

Specificité: Recognizes human CCL13. In sandwich ELISAs, less than 1 % cross-reactivity was observed with rhMCP-1 and no significant cross-reactivity or interference was observed with rhMCP-2, rhMCP-3, r  mMARC, r  mMCP-5, rmJE, rhEotaxin, rhEotaxin-2, rmEotaxin, rhRANTES or rmRANTES.

Purification: Protein G purified

Immunogène: E. coli-derived rhCCL13.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, Flo, Func, Neut

Usage: Neutralization of Bioactivity, Flow Cytometry, ELISA capture. Neutralization of Human CCL13 Bioactivity: The exact concentration of antibody required to neutralize human CCL13 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Flow Cytometry: This antibody was validated for flow cytometry using IL-1 treated A549 cells. For intracellular staining to detect human CCL13, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. ELISA Capture: This antibody can be used as a capture antibody in a human CCL13 ELISA in combination with the biotinylated polyclonal detection antibody. A general protocol is provided on the next page. Using plates coated with 100 μL/well of the capture antibody at 2 μg/mL, in combination with 100 μL/well of the detection antibody, an ELISA for sample volumes of 100 μL can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 0.5 ng/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Détails sur CCL13

Antigène: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Autre désignation: CCL13 / MCP-4

Sujet: Name/Gene ID: CCL13
Family: Intercrine

Synonyms: CCL13, C-C motif chemokine 13, CKb10, MCP-4, MCP4, Monocyte chemotactic protein 4, New CC chemokine 1, Small-inducible cytokine A13, NCC1, CK-beta-10, NCC-1, SCYA13

ID gène: 6357

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Phosphorylation & l'infection par le SRAS-CoV-2