PTH1R anticorps (AA 146-169)

N° du produit ABIN334275

Cet anticorps anti-PTH1R est un anticorps Souris Monoclonal détectant PTH1R dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Boeuf (Vache), Porc, Chien, Singe et Cheval.

Aperçu rapide pour PTH1R anticorps (AA 146-169) (ABIN334275)

Antigène: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Reactivité: Humain, Souris, Boeuf (Vache), Porc, Chien, Singe, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PTH1R est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PTH1R anticorps

Épitope: AA 146-169

Marque: IHC-plus™

Specificité: Recognizes human PTH/PTHrP receptor, MW 80kD, and the receptor dimer, Mr 160kD. A faint nonspecific band at 110kD is also detected. This antibody does not detect the PTH2 receptor.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%) Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%) Opossum, Chicken (92%) Xenopus (88%).

Purification: Protein G purified

Immunogène: Synthetic peptide corresponding to aa146-169 of human PTH/PTHrP Receptor [RRCDRNGSWELVPGHNRTWANYSE]. Species sequence Homology: Mouse & rat (23/24aa). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%), Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%), Opossum, Chicken (92%), Xenopus (88%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Detects PTH/PTHrP receptor in human kidney, vascular smooth muscle and ovary sections. Receptor was also detected in porcine oocytes. Western Blot: 0.5-2 μg/mL. Detects PTH/PTHrP receptor from RIPA lysates of 3T3-L1 pre-adipose fibroblasts. 3T3-L1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P3109-50. Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PTH1R

Antigène: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Autre désignation: PTHR / PTHR1

Sujet: Name/Gene ID: PTH1R
Subfamily: Parathyroid hormone
Family: GPCR

Synonyms: PTH1R, Parathyroid hormone 1 receptor, Parathyroid hormone receptor 1, PFE, Pth-related peptide receptor, PTHR, PTH/PTHr receptor, Pth/pthrp receptor, Parathyroid hormone receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR1, PTHrP-R, PTHrP-receptor

ID gène: 5745

UniProt: Q03431

Pathways: Regulation of Carbohydrate Metabolic Process