IDE anticorps (Internal Region)

N° du produit ABIN334425

Cet anticorps anti-IDE est un anticorps Chèvre Polyclonal détectant IDE dans WB, ELISA et IHC. Adapté pour Humain.

Aperçu rapide pour IDE anticorps (Internal Region) (ABIN334425)

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IDE est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-IDE anticorps

Épitope: Internal Region

Fonction: Insulysin / Insulinase

Séquence: QQYNFDRDNT E

Specificité: This antibody is expected to recognize all reported isoforms (NP_004960.2, NP_001159418.1, NP_001309722.1, NP_001309723.1, NP_001309724.1, NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1

Réactivité croisée: Chien, Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QQYNFDRDNTE, from the internal region of the protein sequence according to NP_004960.1, NP_001159418.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 110-120 kDa band observed in lysates of cell line K562 (calculated MW of 118 kDa according to NP_004960.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur IDE

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Autre désignation: IDE

Sujet: Insulinase, insulysin, insulin-degrading enzyme , FLJ35968, INSULYSIN

ID gène: 3416

NCBI Accession: NP_004960, NP_001159418, NP_001309722, NP_001309723, NP_001309724, NP_001309726

Pathways: SARS-CoV-2 Protein Interactome