MGLL anticorps (Internal Region)

N° du produit ABIN334436

L’anticorps Chèvre Polyclonal anti-MGLL a été validé pour WB, IHC, ELISA, FACS et IF. Il convient pour détecter MGLL dans des échantillons de Humain et Souris.

Aperçu rapide pour MGLL anticorps (Internal Region) (ABIN334436)

Antigène: MGLL (Monoglyceride Lipase (MGLL))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MGLL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-MGLL anticorps

Épitope: Internal Region

Fonction: Monoglyceride Lipase

Séquence: QDLPHLVNAD GQY

Specificité: This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3 kDa according to NP_001003794.1). Recommended concentration: 0.3-1 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur MGLL

Antigène: MGLL (Monoglyceride Lipase (MGLL))

Autre désignation: MGLL

Sujet: Monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like

ID gène: 11343, 23945, 29254

NCBI Accession: NP_009214, NP_001003794

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism